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Typical collen.
Developing collen.
Mature collen.
Thickened corners
Column in cortex
Nuclei
Lamellar, sparse
Lamellar, abundant
Pumpkin petiole
Birch petiole
Leaf vein
Leaf hypodermis

Fig. 4.1-8. Transverse section of stem of elder (Sambucus). This is an unusual sample of collenchyma in that there is a large amount of lamellar collenchyma and it has a large number of intercellular spaces. This particular mass of collenchyma was located below a lenticel, a patch of loose cork cells that permits oxygen to diffuse into the living tissues below the bark. The intercellular spaces in this collenchyma permit oxygen to penetrate to the tissues below. These collenchyma cells have prominent protoplasts (gray; plasmolyzed during processing), and there are so many nuclei (red) present that these must be short cells.

            It is unusual that cell walls are stained red rather than green. If this material was stained with Safranin and Fast Green, then the red color would indicate that these collenchyma cells have lignified their walls. That is fairly common, especially in collenchyma that occurs in long-lived organs like a stem. On the other hand, it may be that this slide was stained with some other, less common stain that gives an unlignified wall a red color rather than a green color.